Modulation of gene expression in rat muscle cells following treatment with nanoceria in different gravity regimes
Giada Graziana Genchi, Andrea Degl'Innocenti, Alice Rita Salgarella, Ilaria Pezzini, Attilio Marino, Arianna Menciassi, Sara Piccirillo, Michele Balsamo, Gianni Ciofani
Oct - 2018
DOI: 10.2217/nnm-2018-0316

journal : Nanomedicine
type: Article Journal

Aim: Oxidative stress (OS) is strictly associated with senescence/pathogenesis of biological systems. As putative countermeasure to environmental OS, cerium oxide nanoparticles (nanoceria [NC]) were administered to muscle cells on ground and aboard the International Space Station. Materials & methods: Transcriptional analyses were conducted through microarray technology and hierarchical clustering. Venn diagram and gene ontology analyses were also performed on selected gene lists. Results: Adaptive responses to both NC administration and to permanence in real microgravity conditions occurred. Enrichment in the biological processes related to aging, body fat development and mesodermal tissue proliferation for NCtreated samples were found. Conclusion: Nanotechnology antioxidants promise applications to pathological conditions governed by OS on Earth and in life-hostile environments (low Earth orbit and deepspace).

keywords : antioxidants cerium oxide nanoparticles H2 afxlamin microarray microgravity skeletal muscle

Notes : Financial & competing interests disclosure. This research has been supported by the Italian Space Agency contract nr. 2016–7-U.0 (NANOROS, Nanotechnology solutions against oxidative stress in muscle tissue during long-term microgravity exposure).