Furnishing spaceship environment: evaluation of bacterial biofilms on different materials used inside International Space Station
Elena Perrin ; Giovanni Bacci ; Laurent Garrelly ; et al. ; - ASI Sponsor
Jul - 2018
DOI: 10.1016/j.resmic.2018.04.001

journal : Research in Microbiology

Volume : 169 ; Issue : 6
type: Article Journal

Abstract
Performed inside International Space Station (ISS) from 2011 to 2016, VIABLE (eValuatIon And monitoring of microBiofiLms insidE International Space Station) ISS was a long-lasting experiment aimed at evaluating the bacterial contamination on different surface space materials subjected to different pre-treatment, to provide useful information for future space missions. In this work, surfaces samples of the VIABLE ISS experiment were analyzed to determine both the total bacterial load (ATP-metry, qPCR) and the composition of the microbial communities (16S rRNA genes amplicon sequencing).Data obtained showed a low bacterial contamination of all the surfaces, with values in agreement with those allowed inside ISS, and with a taxonomic composition similar to those found in previous studies (Enterobacteriales, Bacillales, Lactobacillales and Actinomycetales). No pre-treatment or material effect were observed on both the bacterial load and the composition of the communities, but for both a slight effect of the position (expose/not expose to air) was observed.In conclusion, under the conditions used for VIABLE ISS, no material or pre-treatment seems to be better than others in terms of quantity and type of bacterial contamination.

keywords : International space stationVIABLE ISSMicrobiomeSurfaces

Notes : eValuatIon And monitoring of microBiaLbiofilms insidE ISS (VIABLE ISS) project has been granted by Italian Space Agency (ASI)