Consequences of Simulated Microgravity in Neural Stem Cells: Biological Effects and Metabolic Response
M Silvano ; E Miele ; M Valerio ; et al. ; - ASI Sponsor
Jun - 2015
DOI: 10.4172/2157-7633.1000289

journal : Journal of Stem Cell Research & Therapy

Volume : 2015 ; Issue : 5-6
type: Article Journal

Abstract
Objective: Microgravity was often shown to cause cell damage and impair cell cycle in a variety of biological systems. Since the effects on the neural system were poorly investigated, we aimed to gain insight into how biological processes such as cell cycle, cell damage, stemness features and metabolic status are involved in neural stem cells (NSC) when they experience simulated microgravity. We also wished to investigate whether these modulations were transient or permanent once cells were returned to normal gravity.Methods: NSC were isolated from mouse cerebella and cultured in the Rotary Cell Culture System (RCCS) to model microgravity. We analyzed cell cycle, stress and apoptotic response. We also performed a 1H NMR-based metabolomic analysis and evaluation of stemness features of NSC in simulated microgravity and once in the returned to normogravity cell culture.Results: Biological processes and metabolic status were modulated by simulated microgravity. Cells were arrested in S-phase together with enhanced apoptosis. Metabolic changes occurred in NSC after simulated microgravity. Interestingly, these modulations were transient. Indeed, stemness features and metabolic footprint returned to basal levels after few days of culture in normal conditions. Moreover NSC clonogenic ability was not impaired.Conclusions: Our data suggest that simulated microgravity impacts on NSC biological processes, including cell cycle and apoptosis. However, NSC does not suffer from permanent damage.

keywords : Neural stem cells; Simulated microgravity; Metabolic profile; Cell cycle; Apoptosis

Notes : This work was supported by Italian Space Agency (ASI)